Contents
Description
This help page describes how to retrieve the microarray data for your gene
of interest.
For example, a user is interested in viewing all of the array data for WWOX, a WW
domain-containing gene in human.
Methods for selecting gene-specific microarray data:
Name Details
- Start by logging in to PUMAdb. If you don't have an account, click on "Public Search". If you are a collaborator, then login.
- Search for the gene of interest by clicking on Name Details.
- Specify the search terms: select Homo sapiens from the drop-down menu
and enter WWOX in the Name: field. Query for only one gene symbol, gene
name, or cloneid at a time, and be aware that only the exact, publicly-recognized gene symbol/name or cloneid will work here. You can use wild
cards ('*' or '%') if you are uncertain about your query term or wish to
broaden your search (e.g., WW* if you wish to list all the WW domain-containing genes). If you are unsure about the proper search term (or what
we consider to be the standard), you can also consult SOURCE.
- The Name Details search will return a list of all the spotted clones
that cluster (UniGene) to the WWOX gene (two, in this case). Click any one
of the View expression history links. This link will take you to a program
that displays a histogram of the red/green ratios for spots containing that
clone in all experiments in the database to which you are allowed access.
- Note that even if the search turns up a gene of interest, there is a
possibility that no expression data will be found for it. This results most
often from genes that are represented only on arrays that are not yet publicly
available, but could also result from uniformly poor data for the clone.
Advanced Results Search
This option has the advantage of offering additional search parameters.
For more information about Advanced Results Search, see the PUMAdb Help: Advanced Results Search document.
- After logging in to PUMAdb, click on Advanced Results Search.
- Select Homo sapiens from the Select Organism: menu.
- Select Experimenter, Category, or SubCategory of interest.
- Click on Data Retrieval and Analysis.
- Select the experiment name(s) of interest from the displayed list.
- Click on Data Retrieval and Analysis.
- Under Gene Selection Options select the enter gene names (separated
by '::') radio button. Paste in the cloneids corresponding to the WWOX gene
(i.e., IMAGE:501939::IMAGE:1626898) which were obtained from the previous
Name Details search.
- Under Gene Filtering turn off all filters initially to ensure you get
some results. Do this by clicking off the Use one of: radio button.
'Submit Query' at the bottom of the page.
- On the Clustering page, click on Clustering and Image Generation.
- On the Clustering and Image Generation Page, Submit Query.
- Click on the image at the bottom of the page to see the map details.
Related Help Documents
-
Assigning features to oligos on ChIP-on-chip arrays
